anti rev erba  (Proteintech)

Journal: Cell reports

Article Title: Circadian lncRNA ADIRF-AS1 binds PBAF and regulates renal clear cell tumorigenesis

doi: 10.1016/j.celrep.2022.111514

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit monoclonal anti-Rev-Erba (E1Y6D) , Cell Signaling , Cat#: 13418.

Techniques: Recombinant, Reverse Transcription, Protein Extraction, Transfection, Staining, Protease Inhibitor, In Situ, Labeling, DC Protein Assay, SYBR Green Assay, Immunoprecipitation, Chromatin Immunoprecipitation, Magnetic Beads, RNAscope, Multiplex Assay, Plasmid Preparation, Software

Journal: Xenobiotica; the fate of foreign compounds in biological systems

Article Title: Identification of rhythmic human CYPs and their circadian regulators using synchronized hepatoma cells.

doi: 10.1080/00498254.2020.1737890

Figure Lengend Snippet: Figure 1. Temporal protein expression of circadian clock genes (i.e., BMAL1, E4BP4, DBP, and REV-ERBa) in synchronized HepRG cells. Data are presented as the fold change in clock protein expression normalized to GAPDH and relative to time 0. Data are mean ± SD (n ¼ 5, five separate experiments). p < 0.05 (one-way ANOVA, indicating significant temporal variation in gene expression). RU, relative unit.

Article Snippet: Anti-CYP2C8, anti-E4BP4 and anti-REV-ERBa antibodies were purchased from Proteintech (Chicago, IL).

Techniques: Expressing, Gene Expression

Journal: Xenobiotica; the fate of foreign compounds in biological systems

Article Title: Identification of rhythmic human CYPs and their circadian regulators using synchronized hepatoma cells.

doi: 10.1080/00498254.2020.1737890

Figure Lengend Snippet: Figure 7. REV-ERBa regulates rhythmic CYP2B6 expression in HepRG cells. (A) Schematic representation of a RevRE element in CYP2B6 promoter region (top panel). Effects of REV-ERBa on CYP2B6-Luc reporter activity (bottom panel). Data are mean ± SD (n ¼ 5). p < 0.05 (t test). (B) REV-ERBa represses CYP2B6 mRNA and pro- tein expression. Data are mean ± SD (n ¼ 5). p < 0.05 (t test). (C) Temporal accumulation of CYP2B6 mRNA in REV-ERBa knocked-down or control synchronized HepRG cells. Data are presented as mean ± SD (n ¼ 5). p < 0.05 between two treatments at individual time points (two-way ANOVA and Bonferroni post hoc test). (D) BMAL1 inhibits CYP2B6 mRNA and protein expression. Data are mean ± SD (n ¼ 5). p < 0.05 (t test). Rel, Relative.

Article Snippet: Anti-CYP2C8, anti-E4BP4 and anti-REV-ERBa antibodies were purchased from Proteintech (Chicago, IL).

Techniques: Expressing, Activity Assay, Control

Journal: Xenobiotica; the fate of foreign compounds in biological systems

Article Title: Identification of rhythmic human CYPs and their circadian regulators using synchronized hepatoma cells.

doi: 10.1080/00498254.2020.1737890

Figure Lengend Snippet: Figure 8. REV-ERBa regulate rhythmic CYP2C8 expression in HepRG cells. (A) Effects of REV-ERBa overexpression or knockdown on CYP2C8 mRNA and protein expression. Data are mean ± SD (n ¼ 5). p < 0.05 (t test). (B) Schematic representation of RevRE elements in CYP2C8 promoter region. (C) Temporal accumulation of CYP2C8 mRNA in REV-ERBa knockdown or control synchronized HepRG cells. Data are presented as mean ± SD (n ¼ 5). p < 0.05 between two treatments at individual time points (two-way ANOVA and Bonferroni post hoc test). Rel, Relative.

Article Snippet: Anti-CYP2C8, anti-E4BP4 and anti-REV-ERBa antibodies were purchased from Proteintech (Chicago, IL).

Techniques: Expressing, Over Expression, Knockdown, Control

Journal: Xenobiotica; the fate of foreign compounds in biological systems

Article Title: Identification of rhythmic human CYPs and their circadian regulators using synchronized hepatoma cells.

doi: 10.1080/00498254.2020.1737890

Figure Lengend Snippet: Figure 10. Schematic representation of circadian clock-regulated cycling human CYPs. The E-box binding protein BMAL1 directly controls the rhythmic expression of CYP1A2. Rhythmic expressions of CYP2E1 and CYP3A4 are gener- ated via both E-box and D-box elements. The RevRE binding protein REV-ERBa contributes to rhythmic oscillations in CYP2B6 and CYP2C8. Additionally, BMAL1 negatively regulated the rhythmic expression of CYP2D6 through the DEC2/CEBPa axis (Matsunaga et al., 2012).

Article Snippet: Anti-CYP2C8, anti-E4BP4 and anti-REV-ERBa antibodies were purchased from Proteintech (Chicago, IL).

Techniques: Binding Assay, Expressing